Journal: Children

Article Title: A Novel Variant in the TP53 Gene Causing Li–Fraumeni Syndrome

doi: 10.3390/children10071150

Figure Lengend Snippet: Results of targeted whole exome sequencing: causative variants in the disease genes associated with the reported phenotype.

Article Snippet: WES was performed only on the child, and subsequent Sanger DNA sequencing of the respective locus was performed in six more family members ( ) by GeneDx ( www.geneDx.com , reported on 22 August 2016 for our patient and on 5 May 2017 for the family members).

Techniques: Sequencing, Variant Assay

Journal: BMC Research Notes

Article Title: Validation of differentially methylated microRNAs identified from an epigenome-wide association study; Sanger and next generation sequencing approaches

doi: 10.1186/s13104-018-3872-x

Figure Lengend Snippet: Workflow of analysis methods undertaken in this study. bam binary alignment map, BST bisulphite-treated, CpG cytosine-phosphate-guanine, DKD diabetic kidney disease, gDNA genomic DNA, hg human genome, NGS next generation sequencing, SNP single nucleotide polymorphism, T1D type-1 diabetes mellitus

Article Snippet: Regarding DNA methylation, the BST DNA Sanger sequencing analysis mirrored the methylation sites identified through the Infinium Human Methylation 450K analysis.

Techniques: Next-Generation Sequencing

Journal: BMC Research Notes

Article Title: Validation of differentially methylated microRNAs identified from an epigenome-wide association study; Sanger and next generation sequencing approaches

doi: 10.1186/s13104-018-3872-x

Figure Lengend Snippet: Comparison of SNPs located within miR - 329 - 2 and miR - 429 identified by targeted NGS and Sanger sequencing. The data generated by both platforms showed consistent results for SNP calls. Ion PGM™ data analysed using Partek Genomics Suite is shown on the left, with the complementary Sanger sequence results shown on the right, for matching genomic DNA samples. Chr chromosome, DC diabetic control, DKD diabetic kidney disease, hg human genome, Ref reference, Seq sequence

Article Snippet: Regarding DNA methylation, the BST DNA Sanger sequencing analysis mirrored the methylation sites identified through the Infinium Human Methylation 450K analysis.

Techniques: Comparison, Sequencing, Generated, Control

Journal: Nature Communications

Article Title: Combating acquired resistance to MAPK inhibitors in melanoma by targeting Abl1/2-mediated reactivation of MEK/ERK/MYC signaling

doi: 10.1038/s41467-020-19075-3

Figure Lengend Snippet: Next-generation whole-exome sequencing.

Article Snippet: Sanger sequencing of MAP2K1 in genomic DNA isolated from 451-Lu-BR cells was performed by ACGT, Inc. using the primers described in Supplementary Table , and visualized with 4 Peaks software (free online tool).

Techniques: Sequencing